Difference between revisions of "LB Medium"
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(Created page with "== Info == Used for growing ''E. coli''. == Protocol == # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: #* '''2...") |
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# Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | ||
| − | #* '''25g LB powder''' | + | #* '''25g LB-Miller powder''' *or* '''20g LB-Lennox powder''' *or* '''15.5g LB-Luria powder''' |
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
| Line 17: | Line 17: | ||
If adding antibiotic, add after autoclaving. | If adding antibiotic, add after autoclaving. | ||
| − | 25g of LB powder consists of: | + | 25g of LB-Miller powder consists of: |
| − | + | * 10g tryptone | |
| − | + | * 5g yeast extract | |
| − | + | * 10g NaCl | |
| + | |||
| + | 20g of LB-Lennox powder consists of: | ||
| + | * 10g tryptone | ||
| + | * 5g yeast extract | ||
| + | * 5g NaCl | ||
| + | |||
| + | 15.5g of LB-Luria powder consists of: | ||
| + | * 10g tryptone | ||
| + | * 5g yeast extract | ||
| + | * 0.5g NaCl | ||
Revision as of 02:04, 13 September 2014
Info
Used for growing E. coli.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 25g LB-Miller powder *or* 20g LB-Lennox powder *or* 15.5g LB-Luria powder
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 7.0 using the pH meter and adding drops of NaOH or HCl.
- Make up to 1 litre with water
- Autoclave
Notes
If adding antibiotic, add after autoclaving.
25g of LB-Miller powder consists of:
- 10g tryptone
- 5g yeast extract
- 10g NaCl
20g of LB-Lennox powder consists of:
- 10g tryptone
- 5g yeast extract
- 5g NaCl
15.5g of LB-Luria powder consists of:
- 10g tryptone
- 5g yeast extract
- 0.5g NaCl