Difference between revisions of "Pledge: Biohacking"
| Line 29: | Line 29: | ||
====What we've bought so far:==== | ====What we've bought so far:==== | ||
| − | * | + | *[http://www.nbsbio.co.uk/product.asp?pID=8173&cID=70&c=1507 Agarose 100g] £38 |
| − | * | + | *[http://www.nbsbio.co.uk/product.asp?pID=6241&cID=75 Safewhite 1ml] £30 (This is combined loading dye & DNA stain) |
| − | * | + | *[http://www.nbsbio.co.uk/product.asp?pID=10571&cID=33 DNA Ladder] £15 |
| − | * | + | *[http://nbsbio.co.uk/product.asp?pID=1528&cID=4 TBE Concentrate] £10 |
* Shipping £10 | * Shipping £10 | ||
| Line 39: | Line 39: | ||
...This is everything necessary for [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis of DNA] on an agarose gel, for checking the results of PCR reactions, etc. | ...This is everything necessary for [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis of DNA] on an agarose gel, for checking the results of PCR reactions, etc. | ||
| + | |||
| + | *5-50ul single channel pipette £12 (eBay seller [http://myworld.ebay.co.uk/fizzpinkfizz/ fizzpinkfizz]) | ||
| + | *1000 2-200ul Pipette tips £8.89 (eBay seller [http://myworld.ebay.co.uk/trafalgarscientific/?_trksid=p4340.l2559 trafalgarscientific]) | ||
==PCR machine / Thermal cycler== | ==PCR machine / Thermal cycler== | ||
Revision as of 10:15, 18 April 2011
Gene detection experiment
At a guesstimated total cost of £200.
Together with the equipment that we already have (thermal cycler) and are about to build (electrophoresis tank) this would give us the immediate means to do gene detection (we will design primers to extract particular genes.) E.g.:
- Detection of genetic modifications (identify GM food)
- Detection of species ("lamb curry test": are you getting what you bought?)
- Detection of variants of species (e.g. detect known traits based on published gene sequences)
- We can also send the results to a sequencing lab to get raw data back.
Pledges:
- martind £50
- Andy Cousins (amc) £50
- Gilda £30
- Bugs £50
Details
Estimates
The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
For PCR:
- Primers (£20)
- PCR Reagents (£40)
- 0.5ml tubes (£10),
What we've bought so far:
- Agarose 100g £38
- Safewhite 1ml £30 (This is combined loading dye & DNA stain)
- DNA Ladder £15
- TBE Concentrate £10
- Shipping £10
- VAT £18.60
Total = 121.60
...This is everything necessary for electrophoresis of DNA on an agarose gel, for checking the results of PCR reactions, etc.
- 5-50ul single channel pipette £12 (eBay seller fizzpinkfizz)
- 1000 2-200ul Pipette tips £8.89 (eBay seller trafalgarscientific)
PCR machine / Thermal cycler
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
We won the PCR machine for £120.
Nigel £30- PAID £15Gilda £40- PAID £15Irwin £15- PAID £15Lauren £15- PAID £15Martind £100- PAID £15, pls claim the rest from me once more purchases were madeJonty £15- PAID £15- Radek £30
Bugs £30- PAID £15- Keith $100 USD
Total pledged: £275 + $100 from the colonies
Total needed: £120
PAID: £105
Incubator
This one has been bid on and should be OK, currently at £10 http://cgi.ebay.co.uk/220725833946
...might need a bit of hacking, but we're good at that