Difference between revisions of "Project:Growing bacteria"
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Culture A still ok, culture B almost disappeared. | Culture A still ok, culture B almost disappeared. | ||
Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although ''E. coli'' incubated at 37C for a couple of hours first. | Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although ''E. coli'' incubated at 37C for a couple of hours first. | ||
| − | + | ===18th march, 1pm=== | |
| + | Checked the plates, the two new V. fischeri cultures are glowing very strongly. Can't see much from anything else. | ||
== E. Coli == | == E. Coli == | ||
Revision as of 13:10, 18 March 2013
V. Fischeri
7th march
Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3)
9th march
Some cultures transferred to new plates. Protocol
10th march
More cultures transferred to new plates. Protocol
13th march
Original cultures still glowing strongly, but nothing from the new ones.
15th march
Still nothing from new cultures. Original culture A was still going strong, Culture B is much weaker.
16th march
Culture A still ok, culture B almost disappeared. Two cultures of V. fischeri, two cultures of E. coli, and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of V. fischeri from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although E. coli incubated at 37C for a couple of hours first.
18th march, 1pm
Checked the plates, the two new V. fischeri cultures are glowing very strongly. Can't see much from anything else.
E. Coli
Equipment
We need to fix our incubator with shaker, or make a new one.
Requirements
- Easily cleaned + sterilised
- Either a shaker powerful enough to agitate full beakers, or a magnetic stirrer (which we have already started building). Magnetic stirrer may lyse cells. An idea for a shaker could be a record player.
- Can be made dark inside
- Adjustable temp
- Low power if possible.
- Temperature mesaurement (simple thermometer would do)
- Does it need to be sealable?