Kombucha Medium

Info

Used for growing G. hansenii or kombucha culture.

Protocol

1. Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
   • 100g caster sugar
   • 1 teabag of black or green tea
2. Add around 800ml of 100°C water
3. Remove teabag after 15 minutes
4. Add 100ml cider vinegar measured using a measuring cylinder
5. Mix until all the additives are dissolved
6. Make up to 1 litre with water
7. Autoclave