LBA

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  • You want 100ug/ml ultimately for high copy plasmids, meaning 10mg in 100ml
  • So you can use ampicillin stock solution (1000x conc) at 1ul / ml.

Make new stock

  • Needs to be 1000x, so 100mg per ml

Solubility rating is 50mg/ml, so make 1ml of stock.

Plates

Microwave? Add ampicillin at 48C