LBA

From London Hackspace Wiki
  • You want 100ug/ml ultimately for high copy plasmids, meaning 10mg in 100ml
  • So you can use ampicillin stock solution (1000x conc) at 1ul / ml.

Make new stock

  • Needs to be 1000x, so 100mg per ml

Solubility rating is 50mg/ml, so make 1ml of stock.

Plates

Microwave? Add ampicillin at 48C