Good news - we're open for limited services in Wembley. Ujima House is now actively under refurbishment and we'd love your help in making the space the best it can be.
During this interim period donations and continued membership are greatly appreciated while we transition to our new space.
Mind Controlled Transgene Expression in E. coli
The goal of this project is to mind-control the expression of a recombinant gene in E. coli using a commercial EEG headset. It takes inspiration from the mind-controlled paramecia (@Genspace) and a recent paper on the mind-controlled transgene expression in mammalian cells (Nature comms.)
Types of control
"Neurofeedback" and "direct control" //expand on the definitions in the Nature comms. paper//
IPTG inducible system
This approach consists in the control of an open syringe pump using a commercial EEG headset, based on a simple arduino hack that you can find at this website http://frontiernerds.com/brain-hack The open syringe pump is directly controlled by the arduino, and will only add IPTG to the media when a threshold of concentration/meditation is achieved. The IPTG is added (during log phase growth of the E. coli ) to the media: the bacteria will produce GFP and fluoresce depending on your mind state.
Work so far
- Transformed BBa_K731520 (IPTG inducible GTP, composed of the lacI repressor, lac operator and GFP coding sequence)
- Set up O/N culture IPTG+/-
Hacked Mindflex headset with Arduino to sniff the data.
Ontogenetically controlled system
- Get a better Headset (with this one very low noise/signal ration)
- Complete the genetic circuit for optogenetical mind-control of trangene expression