Protocols/Electroporation
Imperials 2014 iGEM protocol
http://2014.igem.org/Team:Imperial/Protocols
Books on methods
http://www.sciencedirect.com/science/book/9780080917276
Electroporation and Electrofusion of Membranes (D.S. DIMITROV)
Some technical notes on electroporation; http://www.physics.uoguelph.ca/~dutcher/download/handbook%20of%20biological%20physics/18.pdf
Lightning triggered electroporation
http://arxiv.org/ftp/arxiv/papers/1212/1212.5436.pdf
optimizing electroporation
"Electroporation and Electrofusion utilize high voltage, short duration electrical pulses to temporarily create pores in a cell membrane to allow the transfer of molecules or to fuse membranes together. The composition of cell membranes can differ between cell lines. This difference can affect the transfection or transformation parameters needed for successful electroporation. The ability to adjust the parameters using the BTX systems will ensure the highest transfection or transformation rates possible. The following are guidelines for determining ideal electroporation parameters."
http://www.harvardapparatus.com/hapdfs/HAI_DOCCAT_3/W3_9_356.pdf
Pulse generators for electroporation
fairly old doc, but covers some parameters and manufacturers
Design of a MOSFET-Based Pulsed Power Supply for Electroporation
https://uwspace.uwaterloo.ca/bitstream/handle/10012/844/j2grenie2006.pdf?sequence=1
Some circuits for pulsed electric fields
http://altered-states.net/barry/rife/pulsedelectricflds.htm
"This section discusses current knowledge in the application of pulsed electric fields as a method of non-thermal food preservation. It includes mechanisms of inactivation, studies on microbial inactivation, critical process factors, and future research needs. Detailed descriptions of pilot and laboratory-scale equipment and their use in food preservation are also covered."
methods for gene therapy
http://lbk.fe.uni-lj.si/pdfs/beme2004.pdf
There are some notes here
http://openwetware.org/wiki/DIYbio/FAQ/Equipment#Reusing_Electroporation_Cuvettes
""If you have ever worked out the price of an electroporation cuvette you will realise that, at several dollars each, they are worth recycling. Accounts on how amenable electroporation cuvettes are to recycling vary, but I find that as long as you treat them well it is possible to use single cuvette many times. [...] "" See http://bitesizebio.com/2008/04/30/re-cycling-electroporation-cuvettes/
Recycling cuvettes
http://bitesizebio.com/10258/re-cycling-electroporation-cuvettes/
