Anonymous

Pledge: Biohacking: Difference between revisions

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=== Details ===
=== Details ===
====Estimates====


The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
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** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256032 Cole-Parmer, £21.10 for £50]
** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256032 Cole-Parmer, £21.10 for £50]


For [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis]:
====What we've bought so far:====
* Power source (100V, low hundreds of mA; we have this)
*[[http://www.nbsbio.co.uk/product.asp?pID=8173&cID=70&c=1507 Agarose 100g]] £38
* Agarose (£30)
*[[http://www.nbsbio.co.uk/product.asp?pID=6241&cID=75 Safewhite 1ml]] £30 (This is combined loading dye & DNA stain)
* TBE Tablets (£10)
*[[http://www.nbsbio.co.uk/product.asp?pID=10571&cID=33 DNA Ladder]] £15
* Ladder (£20)
*[[http://nbsbio.co.uk/product.asp?pID=1528&cID=4 TBE Concentrate]] £10
* Loading buffer (£20)
* Gel red/SYBR safe (£30)


* Shipping £10
* VAT £18.60
'''Total = 121.60'''


Bugs' quick notes:
...This is everything necessary for [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis of DNA] on an agarose gel, for checking the results of PCR reactions, etc.
NBS biologicals looks good. Cathal has bought from them before, and their prices seem comparable to Sigma, etc.
Getting all of the electrophoresis chemicals from them comes to £107.50, just within the estimate :).
Specifically, product no.s 8970, 8950, 8173, 6241


==PCR machine / Thermal cycler==
==PCR machine / Thermal cycler==
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