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Project:Sex typing with amelogenin/results-20120212: Difference between revisions
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Project:Sex typing with amelogenin/results-20120212 (view source)
Revision as of 17:05, 12 February 2012
, 12 February 2012no edit summary
(Experiment progress) |
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== Gel run 1 == | == Gel run 1 == | ||
# PCR, no master mix, no template, just primers and 55 ul of buffer (Couldn't do this one, ran out of primers) | |||
# PCR, no master mix, just template and primers and 5ul of buffer (Couldn't do this one, ran out of template and primers) | |||
# PCR, master mix and primers, no template and 30 ul of buffer | |||
# PCR, master mix, primers, and template (from top of tube) | |||
# PCR, master mix, primers, and template (from bottom of tube) | |||
# PCR, master mix (old one), primers, and template (from top of tube) | |||
# PCR, master mix (old one), primers, and template (from bottom of tube) | |||
== DNA extraction == | == DNA extraction == | ||
| Line 50: | Line 50: | ||
=== Gel lanes (viewed with the gel oriented so the lanes are at the top) === | === Gel lanes (viewed with the gel oriented so the lanes are at the top) === | ||
# Nothing | |||
# PCR 3 above | |||
# PCR 4 above | |||
# PCR 5 above | |||
# Ladder, 6ul | |||
# PCR 6 above | |||
# PCR 7 above | |||
# Nothing | |||
Started electrophoresis at 16:11 at 100v. | Started electrophoresis at 16:11 at 100v. | ||
