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Project:Sex typing with amelogenin/results-20120212: Difference between revisions
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Project:Sex typing with amelogenin/results-20120212 (view source)
Revision as of 23:10, 12 February 2012
, 12 February 2012Did we do it?
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DNA was extracted following the protocol above: saline solution mouth-wash followed by 10 minutes of immersion in boiling water. | DNA was extracted following the protocol above: saline solution mouth-wash followed by 10 minutes of immersion in boiling water. | ||
[[File:2012-02-12-cell-boiling.jpg|200px|thumb| | [[File:2012-02-12-cell-boiling.jpg|200px|thumb|none|Cell lysis]] | ||
PCR and electrophoresis was performed using the above protocols. | PCR and electrophoresis was performed using the above protocols. | ||
[[File:2012-02-12-biohacking-equipment.jpg|200px|thumb| | [[File:2012-02-12-biohacking-equipment.jpg|200px|thumb|none|Equipment for PCR and electrophoresis (MediaWiki sucks)]] | ||
After 20 minutes, bands were observed in lanes 2, 3, 4, 6, and 7. The ladder was also visible in lane 5 but can't be seen in this photo. | After 20 minutes, bands were observed in lanes 2, 3, 4, 6, and 7. The ladder was also visible in lane 5 but can't be seen in this photo. | ||
[[File:2012-02-12-biohacking-electrophoresis-1.jpg|200px|thumb| | [[File:2012-02-12-biohacking-electrophoresis-1.jpg|200px|thumb|none|After 20 minutes of electrophoresis (MediaWiki sucks)]] | ||
After 45 minutes, the bands had become very blurry. | After 45 minutes, the bands had become very blurry. | ||
[[File:2012-02-12-biohacking-electrophoresis-2.jpg|200px|thumb| | [[File:2012-02-12-biohacking-electrophoresis-2.jpg|200px|thumb|none|After 45 minutes of electrophoresis (MediaWiki sucks)]] | ||
Here is a close-up of the middle portion of the gel. The ladder is faintly visible. | Here is a close-up of the middle portion of the gel. The ladder is faintly visible. | ||
[[File:2012-02-12-biohacking-electrophoresis-2b.jpg|200px|thumb| | [[File:2012-02-12-biohacking-electrophoresis-2b.jpg|200px|thumb|none|After 45 minutes of electrophoresis, close-up (MediaWiki sucks)]] | ||
= Discussion = | |||
* The bands are very blurry and wonky, as if the agar was of varying densities throughout. | |||
* Bands on lanes 3, 4, and 6 travelled less far than bands on lanes 2 and 7. Lane 2 did not include template (i.e. DNA); and I ran out of template in lane 7. So this is an encouraging result. | |||
* The bands are within the bounds of the ladder this time. | |||
* Bands on lanes 3, 4, and 6 are more blurry than bands on lanes 2 and 7. One exciting theory is that bands on those lanes actually worked, and the blurriness is because of the 200 base-pair difference between the two amelogenin sequences; whereas the bands on lanes 2 and 7 contain un-used primer and master mix, which is a) shorter sequences and b) less well-defined (presumably because there are various different lengths). | |||
