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Project:Blood typing: Difference between revisions
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In the UK the distribution of | In the UK the distribution of A, B, AB and O is 42%, 10%, 4% and 44%. | ||
== Process overview == | == Process overview == | ||
1) Obtain two | 1) Obtain two sequences of DNA through PCR, the first containing the deletion site at 258, and the second containing the SNP site at 700 | ||
2) Use restriction enzyme KPNI on the first fragment to cut the O alleles only | 2) Use restriction enzyme KPNI on the first fragment to cut the O alleles only, and use restriction enzyme ALUI on the second fragment to cut the B alleles only. | ||
3) | 3) Do gel electrophoresis on the resulting fragments, hopefully resulting in distinguishable bands to show the 6 different alleles. We should then be able to determine an individual's blood type. | ||
== Process reality | == Process reality == | ||
Much of the necessary equipment we already have from the sex typing experiments. Of the new things, we need the restriction enzymes, and possible a new gel - see below. | |||
All fragments in the papers are between 80 and 200 bp long. For this we would need a polyacrylamide gel (which we have decided against due to difficulty with handling) or a high quality agarose concentrated at 3%, which is a bit expensive, but not impossible. So we are currently looking into the possibility of doing the test using larger fragments. Our agarose at the moment is suitable for fragments > 500bp. | All fragments in the papers are between 80 and 200 bp long. For this we would need a polyacrylamide gel (which we have decided against due to difficulty with handling) or a high quality agarose concentrated at 3%, which is a bit expensive, but not impossible. So we are currently looking into the possibility of doing the test using larger fragments. Our agarose at the moment is suitable for fragments > 500bp. | ||
== New equipment == | |||
Restriction enzymes are listed [here] by sigma: | |||
http://www.sigmaaldrich.com/catalog/search?interface=All&term=restriction+enzymes&lang=en®ion=GB&focus=product&N=0+220003048+219853121+219853286&mode=match%20partialmax | |||
== Sources == | == Sources == | ||
