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Project:Blood typing: Difference between revisions
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1) Obtain two sequences of DNA through PCR, the first containing the deletion site at 258, and the second containing the SNP site at 700 | 1) Obtain two sequences of DNA through PCR, the first containing the deletion site at 258, and the second containing the SNP site at 700 | ||
2) Use restriction enzyme | 2) Use restriction enzyme KPN I on the first fragment to cut the O alleles only, and use restriction enzyme ALU I on the second fragment to cut the B alleles only. | ||
3) Do gel electrophoresis on the resulting fragments, hopefully resulting in distinguishable bands to show the 6 different alleles. We should then be able to determine an individual's blood type. | 3) Do gel electrophoresis on the resulting fragments, hopefully resulting in distinguishable bands to show the 6 different alleles. We should then be able to determine an individual's blood type. | ||
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== New equipment == | == New equipment == | ||
Sigma aldrich have [http://www.sigmaaldrich.com/catalog/product/sigma/r1258?lang=en®ion=GB | Sigma aldrich have [http://www.sigmaaldrich.com/catalog/product/sigma/r1258?lang=en®ion=GB KPN I] and [http://www.sigmaaldrich.com/catalog/product/sigma/r1258?lang=en®ion=GB ALU I]. I couldn't find them on NBS bio. | ||
If we do need high quality agarose sigma has it [http://www.sigmaaldrich.com/life-science/molecular-biology/nucleic-acid-electrophoresis/agarose-and-acrylamide.html here.] | |||
== Sources == | == Sources == | ||
