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Project:Blood typing: Difference between revisions

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Using the ApE software, we have found a possible pair of primers to enable us to use longer fragments.
Using the ApE software, we have found a possible pair of primers to enable us to use longer fragments.
Primers for G deletion sequence:
{|border="1" cellpadding="5"
|-
! scope="col" | Primer
! scope="col" | Sequence
! scope="col" | Tm(°C)
|-
|P1 forward
|17484 5' CCCGCAGGTCCAATGTTGAG 3' 17503
|59
|-
|P2 reverse
|18268 5' ATCTGACAGAGAAGTGACCACG 3' 18247
|58
|}
Primers for G to A SNP:


{|border="1" cellpadding="5"
{|border="1" cellpadding="5"
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