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Project:Plant species testing: Difference between revisions
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We are using primers for plants to test and refine our DNA extraction / PCR technique. First project is to test for peas. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. | We are using primers for plants to test and refine our DNA extraction / PCR technique. First project is to test for peas. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. | ||
== | == Chelex extraction - 17 and 18/7/12 == | ||
Extraction (From [http://www.springerlink.com/content/n6293v8808n5g836/ this] paper - we also have full text available): | Extraction (From [http://www.springerlink.com/content/n6293v8808n5g836/ this] paper - we also have full text available): | ||
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*Saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | *Saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | ||
== | == Precipitation extraction method: 17/7/12, 21/7/12 == | ||
* Blend peas with salt and detergent according to [http://learn.genetics.utah.edu/content/labs/extraction/howto/ this] protocol. Sieve. | * Blend peas with salt and detergent according to [http://learn.genetics.utah.edu/content/labs/extraction/howto/ this] protocol. Sieve. | ||
