Project:Plant species testing: Difference between revisions

Project:Plant species testing (view source)

653 bytes removed , 21 July 2012

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 *Extraction: same procedure, but started with approx 50 mg pea with a disposable pipette tube in 600ul.
-*ElectrophoresisAlso used 0.5% instead of 1% agarose solution.
+*Electrophoresis: same procedure but used 0.5% instead of 1% agarose solution.
-Extraction:
+*Saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for.
-* 'Homogenize' (mush) approx 50 mg pea with a disposable pipette tube in 600ul of 5% chelex solution for 1 min
-* Vortex for 10s then boil for 5 mins, then vortex again for 10s.
-* Centrifuge for 1 min - recover supernatant and use as template
-Electrophoresis:
-* 100ml of agarose solution: 1 gram of agarose powder added to 100ml of 1% TBE solution
-* Microwaved the agarose, cooled to 50 degrees, added 5ul of ethidium bromide stock solution
-* Poured agarose into mould and waited to cool, then covered with 1% TBE.
-* Put ladder into far well, template into near well
-* Electrophoresed for 1 hr at 80V
-* Visualised under UV light through orange filter - saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for.
 == 2nd go: Extraction 17/7/12 ==