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Project:Plant species testing: Difference between revisions
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→Repeat without PCR: 21/7/12
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==== Repeat without PCR: 21/7/12 ==== | ==== Repeat without PCR: 21/7/12 ==== | ||
*Extraction: same procedure, but started with approx 50 mg pea with a disposable pipette tube in 600ul. | |||
*ElectrophoresisAlso used 0.5% instead of 1% agarose solution. | |||
Extraction: | Extraction: | ||
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* Put ladder into far well, template into near well | * Put ladder into far well, template into near well | ||
* Electrophoresed for 1 hr at 80V | * Electrophoresed for 1 hr at 80V | ||
* Visualised under UV light through orange filter - saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | * Visualised under UV light through orange filter - saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | ||
== 2nd go: Extraction 17/7/12 == | == 2nd go: Extraction 17/7/12 == | ||
