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E. coli GFP transformation: Difference between revisions
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(Created page with "= Genetic modification = We're interested in doing genetic modification on micro-organisms at the London Hackspace. == Legal == There are a number of things we have to do in o...") |
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= Genetic modification = | = Genetic modification = | ||
We're interested in doing genetic modification on micro-organisms at the London Hackspace. | We're interested in doing genetic modification on micro-organisms at the London Hackspace. Checklist for GFP experiement: | ||
Freezer. | |||
-20C or colder. | |||
Temperature logger? | |||
Fridge? | |||
Autoclave. | |||
Pressure cooker, electric hob and autoclave tape? | |||
Incubator. | |||
Build or buy. | |||
Cleaning: | |||
Bleach, gloves, paper towels, hand cleaning gel. | |||
Bunsen burner + fuel. | |||
Glassware, pipettes, tools. | |||
Ethanol. | |||
Lots. | |||
To apply for a license we will probably first need: | |||
Written protocols for stuff, especially cleaning up. | |||
Disposal. | |||
Chemical. | |||
Sterilisation. | |||
Do we need incineration? Find a waste disposal company that will take biohazard material? | |||
Restriction enzymes. | |||
At least two. | |||
Pricey? | |||
GFP plasmid. | |||
Antibiotics. | |||
Ampicillin. | |||
Initial e. coli culture. | |||
Growth medium/ broth. | |||
LB. | |||
Plates. | |||
Standard 10cm plates. | |||
Agarose. | |||
Ice. | |||
40C bath. | |||
Proper storage. | |||
Centrifuge | |||
- bigger : hold | |||
Miniprep kit | |||
Maxiprep kit | |||
== Legal == | == Legal == | ||
