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Project:Plant species testing: Difference between revisions
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→Precipitation extraction method: 17/7/12, 21/7/12
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*Saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | *Saw a faint, blurry band, running ahead of the ladder, which we assumed to be the genomic DNA we were looking for. | ||
== Precipitation extraction method: 17/7/12, | == Precipitation extraction method: 17/7/12, 24/7/12 == | ||
* Blend peas with salt and detergent according to [http://learn.genetics.utah.edu/content/labs/extraction/howto/ this] protocol. Sieve. | * Blend peas with salt and detergent according to [http://learn.genetics.utah.edu/content/labs/extraction/howto/ this] protocol. Sieve. | ||
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* Add 70% isopropanol, wait 10 mins for DNA to precipitate. Small amount of DNA visible in isopropanol (We should have done this for longer, possibly in the freezer, to get more DNA) | * Add 70% isopropanol, wait 10 mins for DNA to precipitate. Small amount of DNA visible in isopropanol (We should have done this for longer, possibly in the freezer, to get more DNA) | ||
* Centrifuge for 30 mins to make DNA form pellet at bottom of tube. Unclear if pellet had formed - in future precipitate for longer. | * Centrifuge for 30 mins to make DNA form pellet at bottom of tube. Unclear if pellet had formed - in future precipitate for longer. | ||
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