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Project:Plant species testing: Difference between revisions
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→Chelex extraction method - 17 and 18/7/12
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*Extraction: same procedure, but started with approx 50 mg of pea in 600ul chelex. (This is much more reactant than in the paper and a waste of chelex, but our scale only goes down to 100mg. Would be good to get a smaller one.) | *Extraction: same procedure, but started with approx 50 mg of pea in 600ul chelex. (This is much more reactant than in the paper and a waste of chelex, but our scale only goes down to 100mg. Would be good to get a smaller one.) | ||
*Electrophoresis: same procedure but used 0.5% instead of 1% agarose solution as we are running genomic DNA not PCR product. | *Electrophoresis: same procedure but used 0.5% instead of 1% agarose solution as we are running genomic DNA not PCR product. | ||
*Saw a faint, blurry band, running ahead of the ladder, | *Saw a faint, blurry band, running ahead of the ladder. Not sure what this is, as genomic DNA should be behind the ladder if not still in the well. | ||
== Precipitation extraction method: 17/7/12, 24/7/12 == | == Precipitation extraction method: 17/7/12, 24/7/12 == | ||
