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Project:Plant species testing: Difference between revisions
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→Repeat without PCR: 30/7/12
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==== Repeat without PCR: 30/7/12 ==== | ==== Repeat without PCR: 30/7/12 ==== | ||
* Bashed peas | * Bashed peas into paste in a glove | ||
* | * Placed a sample of template in 250 ul of chelex 5% | ||
* | * Incubate at 56°C for 30 mins, with periodic vortexing, the 94C for 10 mins | ||
* | * 20 ul of supernatant and visualised on 1% agarose gel. | ||
== Precipitation extraction method: 17/7/12, 24/7/12 == | == Precipitation extraction method: 17/7/12, 24/7/12 == | ||
