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Project:Public Biobrick: Difference between revisions
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We loaded 3ul of each template with 2ul of leading buffer (5ul of ladder and 2ul of loading buffer), and electrophoresed on a 1% agarose gel at 76V for 40 mins. | We loaded 3ul of each template with 2ul of leading buffer (5ul of ladder and 2ul of loading buffer), and electrophoresed on a 1% agarose gel at 76V for 40 mins. | ||
[[File: | [[File:Gel_15_9.jpg|left|400px|thumb|none|Gel run 15th sept]] | ||
'''From left:''' | '''From left:''' | ||
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So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. (Not sure yet whether this means PCR succeeded, as 'ANF template' may already have been this size. Will explain what ANF template means when I understand it better myself!). We also got a band from the positive control at the right position (2070bp). | So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. (Not sure yet whether this means PCR succeeded, as 'ANF template' may already have been this size. Will explain what ANF template means when I understand it better myself!). We also got a band from the positive control at the right position (2070bp). | ||
=== Restriction digest for ligation (UCL) === | === Restriction digest for ligation (UCL) === | ||
