Project:Public Biobrick: Difference between revisions

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*7: PSBIC3 PCR product.
*7: PSBIC3 PCR product.


So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. (Not sure yet whether this means PCR succeeded, as 'ANF template' may already have been this size. Will explain what ANF template means when I understand it better myself!). We also got a band from the positive control at the right position (2070bp).
So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. ANF templates were ANF genes that had been previously PCRed with the non prefix/suffix primers. We also got a band from the positive control at the right position (2070bp).


=== Restriction digest for ligation (UCL) ===
=== Restriction digest for ligation (UCL) ===
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