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Project:Public Biobrick: Difference between revisions
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=== Restriction digest and ligation === | === Restriction digest and ligation === | ||
This step involves the insertion of the gene (ANF | This step involves the insertion of the gene (ANF / MR in our case) into the plasmid backbone (PSB1C3). First the plasmid and the gene are incubated in separate reactions with restriction enzymes, to cut them at the appropriate parts so they can be ligated together. | ||
====Restriction digest and ligation 12/9==== | ====Restriction digest and ligation 12/9==== | ||
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Incubated at 37C 30mins, 80C 20mins. | Incubated at 37C 30mins, 80C 20mins. | ||
=====Ligation===== | |||
=== Miniprep: Plasmid DNA Extraction === | === Miniprep: Plasmid DNA Extraction === | ||
TBA | TBA | ||
