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Project:Public Biobrick: Difference between revisions
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=====Ligation===== | =====Ligation===== | ||
'''Ligation''' (Total volume = 20ul) | |||
* Insert - 2ul | |||
* Plasmid - 2ul | |||
* 10xT4 buffer - 2ul | |||
* T4 DNA ligase - 1ul | |||
* dH20 - 13ul | |||
'''Positive control''' (Total volume = 20ul) | |||
* Plasmid control - 2ul | |||
* 10xT4 buffer - 2ul | |||
* T4 DNA ligase - 1ul | |||
* dH20 - 15ul | |||
''Negative control''' (Total volume = 20ul) | |||
* Plasmid control - 2ul | |||
* 10xT4 buffer - 2ul | |||
* dH20 - 16ul | |||
Incubated room temp 10mins, 80C 20mins. | |||
=== Miniprep: Plasmid DNA Extraction === | === Miniprep: Plasmid DNA Extraction === | ||
TBA | TBA | ||
