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Project:Public Biobrick: Difference between revisions
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* dH20 - 16ul | * dH20 - 16ul | ||
Incubated room temp 10mins, 80C 20mins. | Incubated room temp 10mins, ligase heat inactivated at 80C for 20mins. | ||
=== Transformation into ''E.coli'' W3110 cells === | === Transformation into ''E.coli'' W3110 cells === | ||
This step was done at UCL laboratory. | This step was done at UCL laboratory. | ||
