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Project:Public Biobrick: Difference between revisions
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So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. ANF templates were ANF genes that had been previously PCRed with the non prefix/suffix primers. We also got a band from the positive control at the right position (2070bp). | So we got no bands from the O. indofilex gDNA reactions, but we did get bands at the right position (285 bp) from 2/3 of the ANF template ones. ANF templates were ANF genes that had been previously PCRed with the non prefix/suffix primers. We also got a band from the positive control at the right position (2070bp). | ||
[[File:ANF PCR UCL.jpg]] | |||
=== Restriction digest and ligation === | === Restriction digest and ligation === | ||
