Project:Plant species testing: Difference between revisions

Project:Plant species testing (view source)

132 bytes removed , 13 October 2012

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 *3: Isopropanol extraction from 30-31/7
 *4: Isopropanol extraction from 30-31/7
-*5: Sample of liquified pea with no other reagents added apart from
+*5: Sample of liquified pea with no other reagents added apart from autoclaved water.
-autoclaved water.
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+Conclusion: Isopropanol extractions failed because pellet contained too much plant matter - did not filter properly.
-Conclusion: Extraction inadequate again. (Probable cause was that sample contained plant matter, hence pellet was this not DNA. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear.
+Liquified pea gives an easily visible smear. Chelex extraction behaved as last time.
-Email and photos [https://lists.kentgeek.org/pipermail/lhs-biohacking/2012-August/000753.html here].
 ===Repeat without PCR (1/8/12): ===