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E. coli GFP transformation: Difference between revisions
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Maxiprep kit | Maxiprep kit | ||
== Growing competent E. coli == | |||
[http://wiki.london.hackspace.org.uk/w/images/a/af/DIYbio_Collaboration_protocols.pdf Here] is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli, [http://www.sigmaaldrich.com/catalog/product/fluka/l3152?lang=en®ion=GB LB] - £66, 0.1M CaCl2, M9 salts, MgSO4, [http://www.sigmaaldrich.com/catalog/product/sigma/l2025?lang=en®ion=GB bacteriological agar solution], thiamine, D glucose and plates. All links are only the first ones I found, maybe cheaper options. | |||
== Self-cloning == | == Self-cloning == | ||
