927
edits
E. coli GFP transformation: Difference between revisions
From London Hackspace Wiki
→Growing competent E. coli
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
||
| Line 47: | Line 47: | ||
== Growing competent E. coli == | == Growing competent E. coli == | ||
[http://wiki.london.hackspace.org.uk/w/images/a/af/DIYbio_Collaboration_protocols.pdf Here] is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli (cannot find how to buy this yet), [http://www.sigmaaldrich.com/catalog/product/fluka/l3152?lang=en®ion=GB LB] - £66, [http://www.sigmaaldrich.com/catalog/product/sigma/21115?lang=en®ion=GB 0.1M CaCl2] - £32.50, [http://www.sigmaaldrich.com/catalog/product/SIGMA/M6030?lang=en®ion=GB M9 salts] - £57.60, MgSO4, [http://www.sigmaaldrich.com/catalog/product/sigma/l2025?lang=en®ion=GB bacteriological agar solution] - £42.90, [http://www.sigmaaldrich.com/catalog/product/sial/t4625?lang=en®ion=GB thiamine] - ~£20, [http://www.sigmaaldrich.com/catalog/product/sigma/g8769?lang=en®ion=GB D glucose] - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available. | [http://wiki.london.hackspace.org.uk/w/images/a/af/DIYbio_Collaboration_protocols.pdf Here] is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli (cannot find how to buy this yet), [http://www.sigmaaldrich.com/catalog/product/fluka/l3152?lang=en®ion=GB LB] - £66, [http://www.sigmaaldrich.com/catalog/product/sigma/21115?lang=en®ion=GB 0.1M CaCl2] - £32.50, [http://www.sigmaaldrich.com/catalog/product/SIGMA/M6030?lang=en®ion=GB M9 salts] - £57.60, [http://www.sigmaaldrich.com/catalog/product/sigma/m3409?lang=en®ion=GB MgSO4] - £20, [http://www.sigmaaldrich.com/catalog/product/sigma/l2025?lang=en®ion=GB bacteriological agar solution] - £42.90, [http://www.sigmaaldrich.com/catalog/product/sial/t4625?lang=en®ion=GB thiamine] - ~£20, [http://www.sigmaaldrich.com/catalog/product/sigma/g8769?lang=en®ion=GB D glucose] - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available. | ||
== Self-cloning == | == Self-cloning == | ||
