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DNA extraction and precipitation with ethanol / isopropanol: Difference between revisions

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(Created page with "* Get DNA * Add proteinase * Add ethanol * Centrifuge and pour away ethanol * Wash with 70% ethanol * Resuspend in dH20 or TE.")
 
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* Get DNA
* Get DNA
* Add proteinase
* Add 1 part proteinase K to 3 parts sample
* Add ethanol
* Add ethanol
* Centrifuge and pour away ethanol
* Centrifuge and pour away ethanol
* Wash with 70% ethanol
* Wash with 70% ethanol
* Resuspend in dH20 or TE.
* Air dry for ∼10 mins. Can be heated gently.
* Resuspend in dH20 or TE. If the sample isn't dissolving it can be heated at 50-60C to help it.
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