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DNA extraction and precipitation with ethanol / isopropanol: Difference between revisions
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DNA extraction and precipitation with ethanol / isopropanol (view source)
Revision as of 11:57, 25 October 2012
, 25 October 2012no edit summary
Mycoplasma (talk | contribs) (Created page with "* Get DNA * Add proteinase * Add ethanol * Centrifuge and pour away ethanol * Wash with 70% ethanol * Resuspend in dH20 or TE.") |
Mycoplasma (talk | contribs) No edit summary |
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* Get DNA | * Get DNA | ||
* Add proteinase | * Add 1 part proteinase K to 3 parts sample | ||
* Add ethanol | * Add ethanol | ||
* Centrifuge and pour away ethanol | * Centrifuge and pour away ethanol | ||
* Wash with 70% ethanol | * Wash with 70% ethanol | ||
* Resuspend in dH20 or TE. | * Air dry for ∼10 mins. Can be heated gently. | ||
* Resuspend in dH20 or TE. If the sample isn't dissolving it can be heated at 50-60C to help it. | |||
