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Thermal Cycler: Difference between revisions

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Revision as of 12:20, 4 November 2012

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=== Stored files: ===
=== Stored files: ===


==== Program for partially successful PCR run on the [http://wiki.london.hackspace.org.uk/view/Public_Biobrick#14-15.2F9:_Hackspace 14th sept]: ====
* Initial D - 95C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min.
 
* File 60: 96C 5 mins (Initial denaturation) - linked to file 61
* File 60: 95C 30s (Initial denaturation) - linked to file 61
* File 61: 35 cycles of D - 96C 1min A - 55C 30s E - 72C 1min - linked to file 63
* File 61: 30 cycles of D - 95C 15s A - 55C 30s E - 68C 50s (PCR cycles) - linked to file 62
* File 62: 65C 5mins (Final extension) - linked to file 63
* File 63: Soak at 4C
* File 63: Soak at 4C


Mycoplasma
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