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E. coli GFP transformation: Difference between revisions
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→Self-cloning
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[http://www.ncbe.reading.ac.uk/ncbe/protocols/transformer.html Here's] a protocol to do a 'self-cloning' transformation, which we would not need a licence for, and so could do now to practise. | [http://www.ncbe.reading.ac.uk/ncbe/protocols/transformer.html Here's] a protocol to do a 'self-cloning' transformation, which we would not need a licence for, and so could do now to practise. | ||
* | ''Timescale:'' | ||
* | |||
* | * Day 1 - prepare bacterial culture plates, incubate at room temperature in the dark | ||
* | * Before session - make agar plates and refrigerate | ||
* | * Day 5 - Class | ||
* Incubate plates overnight | |||
* Day 6 - Examine results | |||
''Requirements'' | |||
* Some steps state refrigeration is required (E. coli culture on arrival, . Need to check if freezer is ok | |||
* We'll need a fridge to store E. coli culture on arrival, LB plates once made, and culture plates if there's a delay between incubation and visualisation. | |||
''Storage when materials arrive'' | ''Storage when materials arrive'' | ||
* Some must be stored at -20. E. coli should be refrigerated. | * Some reagents must be stored at -20. E. coli should be refrigerated. | ||
* Once E. coli is opened needs to be used in a few days. Implies all needs to be used at once. | * Once E. coli is opened needs to be used in a few days. Implies all needs to be used at once. | ||
* Shipped first class post mon - thurs | * Shipped first class post mon - thurs | ||
