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Thermal Cycler: Difference between revisions
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* If you want to run a method, just run the first file and it will run the whole method for you | * If you want to run a method, just run the first file and it will run the whole method for you | ||
== Stored files: == | |||
Files 1-4 are configurable system files. Linked files run automatically in succession. | |||
* File 1: General soak file, for editing (keep it at a certain temperature for ever) | |||
* File 4: Basic PCR cycle | |||
* Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
* File 60: 96C 5 mins (Initial denaturation) - linked to file 61 | * File 60: 96C 5 mins (Initial denaturation) - linked to file 61 | ||
