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Project:Blood typing: Difference between revisions
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→PCR (Will, Lui, Tom) 12/12
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* Redid PCR on Will's extraction from 8/12 to see if doubling quantity of template helped. PCR 1 was same as last time, PCR 2 was with twice the template and no dH20. First PC was same as last time, second was with twice the template and corresponding less water. | * Redid PCR on Will's extraction from 8/12 to see if doubling quantity of template helped. PCR 1 was same as last time, PCR 2 was with twice the template and no dH20. First PC was same as last time, second was with twice the template and corresponding less water. | ||
* Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
[[File: | [[File:Gel12dec2012.JPG|left|400px|thumb|none|Gel 12 Dec]] | ||
'''From left (after 35 mins at 80V:)''' | '''From left (after 35 mins at 80V:)''' | ||
*1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | *1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | ||
