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E. coli GFP transformation: Difference between revisions
From London Hackspace Wiki
→Genetic modification
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
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Fridge? | Fridge? | ||
Incubator. | Incubator. | ||
Build or buy. | Build or buy. Or could use hackspace environmental chamber. Can be programmed with right temp but non-sterile. | ||
Cleaning: | Cleaning: | ||
Bleach, gloves, paper towels, hand cleaning gel. | Bleach, gloves, paper towels, hand cleaning gel. | ||
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== New lab == | == New lab == | ||
==== Financial feasibility ==== | ==== Financial feasibility of our own space ==== | ||
'''Income:''' | '''Income:''' | ||
* Current membership fee is £8 a month. | * Current membership fee is £8 a month. | ||
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* Reagents. We should work out cost per reaction, assuming an extraction, PCR and electrophoresis. I estimate this will be between £1 and £5 per reaction. | * Reagents. We should work out cost per reaction, assuming an extraction, PCR and electrophoresis. I estimate this will be between £1 and £5 per reaction. | ||
So we have a big funding shortfall. | So we have a big funding shortfall. Therefore we need to stay with the hackspace. | ||
''Funding sources:'' | ''Funding sources:'' | ||
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* NCBE | * NCBE | ||
==== Requirements for lab ==== | ==== Requirements for lab + HSE risk assessment ==== | ||
* Tiling / washable surfaces | * Tiling / washable surfaces | ||
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* Lockable | * Lockable | ||
* Enough room for 10-15 people to work | * Enough room for 10-15 people to work | ||
* Disposal procedures | |||
