927
edits
E. coli GFP transformation: Difference between revisions
From London Hackspace Wiki
→Growing competent E. coli
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
||
| Line 52: | Line 52: | ||
== Growing competent E. coli == | == Growing competent E. coli == | ||
[http://wiki.london.hackspace.org.uk/w/images/a/af/DIYbio_Collaboration_protocols.pdf Here] is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli ([http://www.bio-rad.com/prd/en/US/LSE/SKU/166-0408EDU/E.-coli-Strain-HB101-K-12 one possibility]), [http://www.sigmaaldrich.com/catalog/product/fluka/l3152?lang=en®ion=GB LB] - £66, [http://www.sigmaaldrich.com/catalog/product/sigma/21115?lang=en®ion=GB 0.1M CaCl2] - £32.50, [http://www.sigmaaldrich.com/catalog/product/SIGMA/M6030?lang=en®ion=GB M9 salts] - £57.60, [http://www.sigmaaldrich.com/catalog/product/sigma/m3409?lang=en®ion=GB MgSO4] - £20, [http://www.sigmaaldrich.com/catalog/product/sigma/l2025?lang=en®ion=GB bacteriological agar solution] - £42.90, [http://www.sigmaaldrich.com/catalog/product/sial/t4625?lang=en®ion=GB thiamine] - ~£20, [http://www.sigmaaldrich.com/catalog/product/sigma/g8769?lang=en®ion=GB D glucose] - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available. | [http://wiki.london.hackspace.org.uk/w/images/a/af/DIYbio_Collaboration_protocols.pdf Here] is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli ([http://www.bio-rad.com/prd/en/US/LSE/SKU/166-0408EDU/E.-coli-Strain-HB101-K-12 one possibility]. Could also try NCBE. They sell e. coli as a transformer kit replacement part), [http://www.sigmaaldrich.com/catalog/product/fluka/l3152?lang=en®ion=GB LB] - £66, [http://www.sigmaaldrich.com/catalog/product/sigma/21115?lang=en®ion=GB 0.1M CaCl2] - £32.50, [http://www.sigmaaldrich.com/catalog/product/SIGMA/M6030?lang=en®ion=GB M9 salts] - £57.60, [http://www.sigmaaldrich.com/catalog/product/sigma/m3409?lang=en®ion=GB MgSO4] - £20, [http://www.sigmaaldrich.com/catalog/product/sigma/l2025?lang=en®ion=GB bacteriological agar solution] - £42.90, [http://www.sigmaaldrich.com/catalog/product/sial/t4625?lang=en®ion=GB thiamine] - ~£20, [http://www.sigmaaldrich.com/catalog/product/sigma/g8769?lang=en®ion=GB D glucose] - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available. | ||
== Self-cloning == | == Self-cloning == | ||
