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Project:Blood typing: Difference between revisions
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[[Category:Biohacking]] | [[Category:Biohacking]] | ||
==Extraction and PCR (Will, Simon, Lui) 12/2/13== | |||
* Lui extracted DNA from his cheek using [http://biology.clc.uc.edu/fankhauser/Labs/Genetics/Buccal_DNA_isolation/buccal_dna_images_index.html this] protocol. | |||
* Ran PCR with PB2 primers on this + 3 RCA extractions + a positive control. | |||
* 25ul total PCR reaction volume - 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix, 2.5ul dH20 (6.5ul for PC) | |||
* Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | |||
[[File:gel2feb2013.JPG|left|400px|thumb|none|Gel 12 Feb]] | |||
* 15ul of samples loaded, 6ul of ladder loaded. | |||
'''From left (after 40 mins at 80V:)''' | |||
*1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. Used 7ul. | |||
*2: Lui PCR product | |||
*3: Pip PCR product | |||
*4: A PCR product | |||
*5: J PCR product | |||
*6: PC PCR preduct | |||
*7: Lui gDNA | |||
*8: Pip gDNA | |||
*9: A gDNA | |||
*10: J gDNA | |||
<br style="clear: both" /> | |||
'''Conclusions:''' | |||
