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Project:Blood typing: Difference between revisions
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→Extraction and PCR (Will, Simon, Lui) 12/2/13
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'''From left (after 40 mins at 80V:)''' | '''From left (after 40 mins at 80V:)''' | ||
*1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | *1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | ||
*2: Lui PCR product | *2: Lui PCR product (nothing) | ||
*3: Pip PCR product | *3: Pip PCR product (nothing) | ||
*4: A PCR product | *4: A PCR product (band) | ||
*5: J PCR product | *5: J PCR product (faint band) | ||
*6: PC PCR | *6: PC PCR product (band) | ||
*7: Lui gDNA | *7: Lui gDNA (nothing) | ||
*8: Pip gDNA | *8: Pip gDNA (band) | ||
*9: A gDNA | *9: A gDNA (faint band) | ||
*10: J gDNA | *10: J gDNA (band) | ||
<br style="clear: both" /> | <br style="clear: both" /> | ||
'''Conclusions:''' | '''Conclusions:''' | ||
* Lui's extraction didn't contain enough liquid after boiling step, so he added more water afterwards and mixed. This could be responsible for extraction failure. | |||
