927
edits
Project:Blood typing: Difference between revisions
From London Hackspace Wiki
→Extraction and PCR (David, Victor, Will) 17/2/13
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
||
| Line 585: | Line 585: | ||
* David and Will extracted DNA from cheeks using [http://biology.clc.uc.edu/fankhauser/Labs/Genetics/Buccal_DNA_isolation/buccal_dna_images_index.html this] protocol. | * David and Will extracted DNA from cheeks using [http://biology.clc.uc.edu/fankhauser/Labs/Genetics/Buccal_DNA_isolation/buccal_dna_images_index.html this] protocol. | ||
* Ran PCR with PB1 primers on this + Victor's extraction from 13/2 + a positive control. | * Ran PCR with PB1 primers on this + Victor's extraction from 13/2 (same protocol) + a positive control. | ||
* 25ul total PCR reaction volume - 2.5ul dH20 (6.5ul for PC), 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix | * 25ul total PCR reaction volume - 2.5ul dH20 (6.5ul for PC), 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix | ||
* Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
