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Project:Blood typing: Difference between revisions
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→Extraction and PCR (Simon, Lui, Will) 26/2/13
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* Incubated JJ's PCR product with Kpn1 at 37C for 65 mins. Restriction digest mix contained 25ul PCR product, 1ul Kpn1, 15ul buffer. | * Incubated JJ's PCR product with Kpn1 at 37C for 65 mins. Restriction digest mix contained 25ul PCR product, 1ul Kpn1, 15ul buffer. | ||
* Ran result on a gel. PCR product remained undigested. | * Ran result on a gel. PCR product remained undigested. | ||
'''Notes/Conclusions:''' | |||
* We'll need to vary the quantities of the restriction digest to try and get better results. | |||
