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=== 7th march === | === 7th march === | ||
Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3) | Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3) | ||
[[File:Glowing_plates.jpg|200px|thumb|left|Photo by Raphael Kim]] | |||
=== 9th march === | === 9th march === | ||
Some cultures transferred to new plates. [http://wiki.london.hackspace.org.uk/view/File:Vibrio_Fischeri_Culture_Protocol_9.3.pdf Protocol] | Some cultures transferred to new plates. [http://wiki.london.hackspace.org.uk/view/File:Vibrio_Fischeri_Culture_Protocol_9.3.pdf Protocol] | ||
=== 10th march === | |||
More cultures transferred to new plates. [http://wiki.london.hackspace.org.uk/view/File:10.3.13.pdf Protocol] | |||
[[File:V.fisch_culturing.JPG|200px|thumb|left|Photo by Raphael Kim]] | |||
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===13th march=== | ===13th march=== | ||
Original cultures still glow, but nothing from the new ones | Original cultures still glow, but nothing from the new ones | ||
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