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Project:Growing bacteria: Difference between revisions
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Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although ''E. coli'' incubated at 37C for a couple of hours first. | Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although ''E. coli'' incubated at 37C for a couple of hours first. | ||
===18th march, 1pm=== | ===18th march, 1pm=== | ||
[[File:18 March_glowing plates.JPG|200px|thumb|left|Photo by Millie Clive-Smith]] | |||
Checked the plates, the two new V. fischeri cultures are glowing very strongly on the first areas streaked. Can't see much from anything else. | Checked the plates, the two new V. fischeri cultures are glowing very strongly on the first areas streaked. Can't see much from anything else. | ||
===22th march=== | ===22th march=== | ||
3 new plates of V. fischeri made up | 3 new plates of V. fischeri made up | ||
