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Project:Growing bacteria: Difference between revisions
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→V. fischeri + E. coli
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=== V. fischeri life cycle + plating techniques === | === V. fischeri life cycle + plating techniques === | ||
* Peak brightness on day 2-4. Faint by day 5. Faint glow can remain until day 8. | * Apparently they glow brighter in the evening. | ||
* So far no frozen cultures have been revived | * No or faint brightness on day 1. Peak brightness on day 2-4. Faint by day 5. Faint glow can remain until day 8. | ||
* | * So far no frozen cultures have been revived. | ||
* BOSS media still works after being frozen. | |||
* Media can be boiled. | |||
* Recipe: 2% table salt, 2.5% LB, 1.5% agar. For 100ml of BOSS media this would be 2g table salt, 2.5g LB, 1.5g agar in 100ml dH20. | * Recipe: 2% table salt, 2.5% LB, 1.5% agar. For 100ml of BOSS media this would be 2g table salt, 2.5g LB, 1.5g agar in 100ml dH20. | ||
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===20th April 18:00 === | ===20th April 18:00 === | ||
* A14Apr and B14Apr not glowing, A20apr and b20apr not glowing. Made A21apr from A14apr and frozen 20apr BOSS, B21apr from A20apr and fresh BOSS. | * A14Apr and B14Apr not glowing, A20apr and b20apr not glowing. Made A21apr from A14apr and frozen 20apr BOSS, B21apr from A20apr and fresh BOSS. | ||
===22nd April 19:00 === | |||
* A14Apr and B14Apr not glowing, A20apr and b20apr glowing strongly. A21apr from A14apr glowing. | |||
== Equipment == | == Equipment == | ||
