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Transforming bacteria: Difference between revisions
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5) 37°C incubator for one hour | 5) 37°C incubator for one hour | ||
6) 3x200µl of plated on to LB amp plates, incubated overnight 37°C. | 6) 3x200µl of plated on to LB amp plates, incubated overnight 37°C. | ||
===Replating Wed 4 sept 2013=== | |||
* Replated colonies from DH5α plate on one amp plate, DH5alpha; colonies transformed with PUC19 on another. Incubated at 37C overnight. | |||
