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Transforming bacteria: Difference between revisions
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→Replating Wed 4 sept 2013
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6) 3x200µl of plated on to LB amp plates, incubated overnight 37°C. | 6) 3x200µl of plated on to LB amp plates, incubated overnight 37°C. | ||
=== | ===Transformation Wed 11 sept 2013=== | ||
* | * Incubated 50ul of competent cells from 9-11 with 2ul PUC19 for 30 mins on ice (also control with no PUC19 added). Then 42C for 45 seconds. 2 mins back on ice. Added 500ul LB. Incubated at 37C for 1 hr. Plated on amp plates and incubated at 37C overnight. | ||
