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Project:Alcohol Flush Genotyping: Difference between revisions
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Project:Alcohol Flush Genotyping (view source)
Revision as of 17:37, 13 October 2013
, 13 October 2013→SNP genotyping
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GAGAGACTTC AGGGGGCGGA GCGGAGAGGA AAAGCTTCTA GTAAGAATCT TTTCAGATTT | GAGAGACTTC AGGGGGCGGA GCGGAGAGGA AAAGCTTCTA GTAAGAATCT TTTCAGATTT | ||
TCACCAGGCG CGGTGGCTTT | TCACCAGGCG CGGTGGCTTT | ||
=== Tetra-primers === | |||
Suggested primers for tetra=primer PCR from [http://primer1.soton.ac.uk/primer1.html tetra-primer designer]: | |||
Forward inner primer (A allele): Melting temperature | |||
474 GGCGAGTACGGGCTGCAGGCATACAATA 501 74 | |||
Reverse inner primer (G allele): | |||
530 AGCAGGTCCCACACTCACAGTTTTCACGTC 501 74 | |||
Forward outer primer (5' - 3'): | |||
238 GCAACTCCAGCCTGGGCAACAGAGAAA 264 74 | |||
Reverse outer primer (5' - 3'): | |||
669 GTCCTGAACTTCCAGCAGGCCCTGAGTC 642 74 | |||
Product size for A allele: 197 | |||
Product size for G allele: 293 | |||
Product size of two outer primers: 432 | |||
== Links == | == Links == | ||
