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Project:Sex typing with amelogenin: Difference between revisions
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Project:Sex typing with amelogenin (view source)
Revision as of 22:46, 24 April 2014
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== 22nd Feb 2012 == | == 22nd Feb 2012 == | ||
Tonight we did DNA extraction following the protocols from the 15th -- 4 people did a meat tenderiser and alcohol-based extraction, and 3 did a heating and Chelex-based extraction. | |||
We followed the recommendations from bugs for primer strength and overall reaction volume (see the notes from the 15th). | |||
We set the thermocycler to the program mentioned in a paper which used the same primers (see notes from the 12th): | |||
Denature: 1 minute at 94 C; Anneal: 2 minutes at 65 C; Extend: 3 minutes at 72 C. | |||
Unfortunately we ran out of time and did not wait for the thermocycler to complete for visualisation. we will do that next week. | |||
== 29th Feb 2012 == | == 29th Feb 2012 == | ||
