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Project:Sex typing with amelogenin: Difference between revisions
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Project:Sex typing with amelogenin (view source)
Revision as of 00:00, 25 April 2014
, 25 April 2014→21st March 2012 : Success!!
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== 21st March 2012 : Success!! == | == 21st March 2012 : Success!! == | ||
[[/ | Following [http://www.jicef.or.jp/wahec/ful318.htm this chelex protocol] | ||
* Chelex 5ul | |||
* PCR at 96x1 58x1 72x1 @ 35 cycles - with initial denat at 94 for 2 mins | |||
* Oil on chelex durin incubation | |||
* 8ul of 2.5uM primer (4ul of each, mixed) | |||
[[File:GEL_fuck_yes.jpg|400px|thumb|none|The moment of truth]] | |||
[https://lists.kentgeek.org/pipermail/lhs-biohacking/2012-March/000299.html The announcement on the mailing list] | |||
== 22nd March 2012: Anti-success == | == 22nd March 2012: Anti-success == | ||
