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Chelex extraction: 35 cycles of D - 96° for 1:00, A - 56° for 0:30, E - 72° for 1:00. Good result. Detailed info [https://lists.kentgeek.org/pipermail/lhs-biohacking/2012-April/000352.html here]. | Chelex extraction: 35 cycles of D - 96° for 1:00, A - 56° for 0:30, E - 72° for 1:00. Good result. Detailed info [https://lists.kentgeek.org/pipermail/lhs-biohacking/2012-April/000352.html here]. | ||
The major problem seems to be quality of the extracted DNA: PCR product is frequently quite faint. As of April 26, 2012, DNA extraction technique will be the main focus for the amelogenin test (and for genetic testing of human DNA in general). | |||
== 16th May 2012 == | == 16th May 2012 == | ||