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(4) As a first step, we could put the split-gfp alone into a plasmid and transform that into our K-12 as a negative control (as we do not expect it to glow). Such a plasmid could be a useful general first step for other work, and may represent an improvement on previous work. It may also be covered under self-cloning exceptions. | (4) As a first step, we could put the split-gfp alone into a plasmid and transform that into our K-12 as a negative control (as we do not expect it to glow). Such a plasmid could be a useful general first step for other work, and may represent an improvement on previous work. It may also be covered under self-cloning exceptions. | ||
[[Category:Biohacking]] | |||